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T7193
Sigma
Trizma® Pre-set crystals
pH 7.0, average Mw 154.8
| Synonym: | Tris crystals |
Description
| Application | Pre-mixed combinations of TRIZMA Base and TRIZMA HCl to provide the most common pH values for Tris buffers. Dissolve in water and use, no pH adjustment is necessary. Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature. Guaranteed accuracy: ±0.1 pH units. If a different concentration is desired, note the concentration effect: a 0.5 M solution will have a pH 0.05 units higher; a 0.005 M solution will have a pH 0.05 units lower. For buffers in pre-weighed foil pouches, dissolve one packet in 900 mL of water; add additional water to 1 liter to make a 100 mM solution. Temperature: 25 oC. |
| Other Notes | Certain pH electrodes do not give accurate results with Tris buffers. |
| Legal Information | Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | Biotechnology Performance Certified |
| mol wt | average Mw 154.8 |
| total impurities | DNase, RNase, Protease, none detected |
| bioburden, tested | |
| endotoxin, tested | |
| ≤0.05 A290 | |
| ≤0.5% water (Karl Fischer) | |
| ≤5 ppm Heavy metals (as Pb) | |
| pH | 6.70 (37 °C, 0.05 M)(lit.) |
| 7.00 (25 °C, 0.05 M)(lit.) | |
| 7.0 | |
| 7.55 (5 °C, 0.05 M)(lit.) | |
| useful pH range | 7.0 - 9.0 |
| suitability | cell culture tested |
Safety
| WGK Germany | 2 |
